- Dona Paula, Goa, India.
- +91-0832- 2450327
- iiosc2020[at]nio[dot]org
| Abstract Submission No. | ABS-2022-05-0063 |
| Title of Abstract | Marine sponge associated bacteria exhibit protease inhibitory potential. |
| Authors | Maushmi Kumar* |
| Organisation | SVKM'S NMIMS, SPPSPTM |
| Address | SPPSPTM, SVKM'S NMIMS, Vile Parle west, Mumbai, Maharashtra, India Pincode: 400056 Mobile: 9930833575 E-mail: maushmiskumar@gmail.com |
| Country | India |
| Presentation | Oral |
| Abstract | In order to aim for sustainable production of bioactive molecules from marine source, we have isolated marine sponge associated bacteria and fungi species from Ratnagiri coast, Maharashtra. We have checked the effects of sea water- biotic and abiotic parameters on sponge growth and their associated culturable microorganisms. Studies were conducted on triturated sponge samples to analyze the number of culturable bacteria, vibrio and heterotrophic bacteria present by dilution and plating method. Spongia obscura was found to show the highest number of culturable bacteria (1200 CFU/ml), while Haliclona cratera and Hyortis cavernosus inhabited highest number of vibrio (850 CFU/ml) and heterotrophic bacteria (1240 CFU/ml) respectively. Seawater analysis values obtained for the fecal coliform analysis gave evidence to the presence of anthropogenic contamination present in the seawater samples. The study of temporal and spatial distribution of the pH, temperature and salinity were found to be in the same range as that of the waters of Bhayate Estuary, Ratnagiri. The voltage and resistivity were recorded for the first time. The conductivity, total dissolved solids and dissolved oxygen values showed a wide variety of variations and did not match the parameter results when compared to the Bhayate Estuary, Ratnagiri and the water bodies of Gujarat. The nutrient content of the collected surface seawater showed highest nitrate content in the month of January and December, which corresponds to good growth of sponge. Phosphate and silicate analysis conducted showed highest values during the month of March. The conditions for the growth of the sponges particularly pH (6.8-8.2), temperature (22-24⿿), salinity (25-53ppt) and dissolved oxygen (295-692.2mg/l) supported maximum growth of sponges and matched the value range mentioned in earlier studies, while the rest of the parameters showed variation from the compared literature. For biological screening, bacteria and fungi were isolated from Sarcotragus foetidus, Haliclona cratera, Xestospongia carbonaria and Hyrtios cavernosus and purified for screening of their antimicrobial and protease inhibitory activities. We could isolate 8 actinomycetes solely from H. cavernosus, 118 bacteria and 40 fungi isolates from remaining three sponges. Primary screening with caseionolytic plate assay and tryptic digestion assay guided selection of three strains to show good protease inhibitory activity. They were identified as Bacillus subtilis subsp. subtilis NCIB 3610(T) and Aspergillus awamori from H. cratera, Aerococcus virus ATCC 11563(T) from Hyrtios cavernosus by 16S and 18S rRNA sequencing as protease inhibitor producing strains. They gave 26.38 %, 46% and 100% trypsin inhibitory activity. The isolated strains sequences are submitted in Genbank with accession numbers MN482715, MN396261 and MN482715 respectively. The samples were purified by ammonium sulphate precipitation and gel filtration chromatography and fractions are under study for identification of bioactive protein. We aim to determine and identify the protease inhibitor from these sponges associated microorganisms for exploring them as antiviral agents in future and scale up the bioprocess. |