- Dona Paula, Goa, India.
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| Abstract Submission No. | ABS-2022-05-0464 |
| Title of Abstract | 16S Microbiome profiling of the water column from Polymetallic Nodulerich region in the Central Indian Ocean Basin |
| Authors | Shruti Shah*, Samir R. Damare, Vruti Naik, Jayesh Patil, Sneha Parab, Sushil Nair, Brenda Mascarenhas |
| Organisation | Biological Oceanography Division, CSIR-National Institute of Ocea |
| Address | CSIR-National Institute of Oceanography Dona Paula, Goa, India Pincode: 403 004 Mobile: 0 E-mail: sshruti286@gmail.com |
| Country | India |
| Presentation | Oral |
| Abstract | Polymetallic nodules are metal-rich deposits found on the seabed in certain regions of the Pacific and Indian Ocean. These sites are potential mining sites for extraction of these mineral deposits. This study aimed to decipher the microbial diversity from the water column of polymetallic nodule-rich region of the Central Indian Ocean Basin. A total of 20 water samples were obtained across three stations [BC20 (7), IRZ (7) and PRZ (6)] along with the physicochemical parameters at each sampled depth. The average water column depth was 5100 m. A comparison between the samples of the same station and across stations elucidated a total of 28,812 OTUs with maximum OTUs (2120) at PRZ (3500 m). The total OTUs obtained were classified into 49 phyla, 128 classes, 328 orders, 621 families, 1308 genera and 1743 species. A higher species diversity was observed below 600 m depth in the water column. Proteobacteria was found to be the most dominant bacterial phylum throughout the water column followed by Cyanobacteria with higher abundance only at 30 m and chlorophyll maxima. Actinobacteria, Marinimicrobia (SAR 406 clade), Firmicutes, Bacteroidetes, Planctomycetes, Chloroflexi and Verrucomicrobia are some of the other abundant bacterial phyla. Among Archaea, Euryarchaeota was found to be the most abundant phylum followed by Thaumarchaeota. Crenarchaeota group was found only in the lower depth of the water column while it was not detected above 200 m. The lower depths (3500 and 5100 m) showed a positive correlation with total phosphate, nitrate and suspended particulate matter while the 30 m, chlorophyll maxima, 200m and 1000 m showed a positive correlation with dissolved oxygen, pH and temperature. A negative correlation of the environmental parameters with 600 m depth was observed. Microbial groups such as Nitrospira, Nitrospinia, Dehalococcoidia, Actinobacteria, Bacilli, Deferribacteres and Nitrososphaeria were present with higher abundance below 200 m depth and positively correlated with the nitrate, phosphate and suspended organic matter indicating their significance in nutrient cycling and biogeochemical cycles especially Nitrogen cycle in the deep sea environment. |